Term
| What are 8 different protein products produced in bacillus hosts, in addition to corresponding average yields |
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Definition
alpha amylase
interferon
lipase
penicillin G
cellulase
gelatin |
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Term
| what is the percentage of industrial enzymes produced in bacillus hosts? |
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Definition
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Term
| List 8 bacillus strains used for industrial protein expression? where can the genome sequence of these strains be found? |
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Definition
B. subtilis
B. licheniformins
B. amyloliquifaciens
B. thuringiensis |
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Term
| heterologous protein expression occurs during _______ |
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Definition
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Term
What is the diagram illustrating in relation to manufacturing and secreting recombinant enzymes?
At what point is the vegetative cell cycle is sporulation initiated and under what conditions? |
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Definition
| initiated towards the end of the vegetative cell cycle when the cell has 2 complete copies of the chromosome |
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Term
| How does bacillus respond to environmental stress (list 4 environmental stressors) |
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Definition
| oxidative stress, heat stress, phosphate starvation, glucose starvation |
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Term
| Where are the components of vectors used for bacillus subtilis derived from? |
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Definition
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Term
| What structure is provided as well as elements used for xylose-inducible recombinant gene expression and for plasmid replication in bacillus and e coli |
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Definition
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Term
| What are 4 general characteristics of the T7 expression system for bacillus? |
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Definition
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Term
| What are the 4 uses of the pMutin vectors in a chromosomal integration system? |
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Definition
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Term
| What are the methods used to introduce heterologous genes into bacillus hosts? |
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Definition
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Term
| what happens to integrants when introducing heterolgous genes into bacillus hosts by recombination? what are 2 examples of introducing heterologous genes into bacillus? |
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Definition
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Term
| what are 7 advantages of protein expression in bacillus hosts? |
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Definition
1. GRAS
2. does not contain endotoxin
3. high protein secretion capacity
4. industrial producer of secreted peptides
5. relatively insenstive to codon bias
6. pproduces peptide antibiotices
7. can be stored as spores |
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Term
| What are 7 disadvantages of protein expression in bacillus hosts? |
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Definition
|
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Term
| what factors are important when optimizing bacillus for secretion of heterolgous biopharmaceutical proteins? |
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Definition
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Term
| What are 4 characteristics of the expression systems for bacillus? |
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Definition
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Term
| What affects the yield of expressed and secreted proteins in bacillus? |
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Definition
misfolded proteins
degradation by proteases
maturation
jamming |
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