Term
| What are the steps of tissue preparation? |
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Definition
Felix Died Eating Sushi and Maki
Fixation
Dehydration
Embedding
Sectioning
Mounting |
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Term
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Definition
| Using chemicals to preserve the normal structure of the tissue |
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Term
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Definition
replacing the tissue water with increasing concentrations of alcohol.
Make transparent with xylene |
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Term
What is the role of xylene? |
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Definition
| Make the tissue transparent |
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Term
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Definition
| Infiltrate tissue with parafin |
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Term
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Definition
| Slicing the tissue into thin slices |
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Term
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Definition
| placing sections on glass slides with coverslip and staining them |
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Term
| What are the two types of stain? |
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Definition
Hematocyanin (Basic, Blue)
Eosin (Acidic, Red) |
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Term
| List the basic tissue types |
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Definition
| Epithelium, Connective, Muscle, Nervous |
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Term
| How many types of epithelium are there? |
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Definition
Surface
Glandular (Exocrine, Endocrine) |
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Term
| What are the three main types of microscopy? |
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Definition
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Term
| Whats the difference between SEM and TEM? |
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Definition
SEM- surface features are highly detailed
TEM- internal organelles in high resolution |
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Term
| What are the main characteristics of epithelium? |
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Definition
closely adhering cells (tight junctions)
avascular
supported by CT |
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Term
| What are the functions of epithelium? |
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Definition
protection of underlying organs and structures
transport material between tissues and organs
Absorbtion |
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Term
| What are the two types of surface epithelium? |
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Definition
endothelium (lines vessels, and lymphatics)
mesothelium (lines pleural, pericardial, and preitoneal cavity) |
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Term
| How can epithelium be classified? |
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Definition
Number of layers: simple vs stratified
Shape of cells: columnar, cuboidal, squamous
Keratinized vs non-keratinized |
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Term
| Where can simple squamous epithelium be found? |
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Definition
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Term
| Where can simple cuboidal epithelium be found? |
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Definition
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Term
| Where can simple columnar epithelium be found? |
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Definition
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Term
| What are the characteristics of pseudostratified epithelium? |
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Definition
appears stratifued, but all cells touch B.M
Contain tufts of microvilli/cilia |
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Term
| Describe the surface polarity of an epithelia |
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Definition
Apical-side facing surface (lumen)
Lateral side- side that contains junctions. separates basal from apical.
Basal surface- omteracts wotjbasal lamina (b.M)
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Term
| what is transitional epithelium? |
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Definition
| contain dome cells which can stretch and relax to change conformation |
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Term
| Describe the structure of microvilli |
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Definition
Cytoplasmic projections into the apical surface
Bundles of actin are held together by fimbrin
Actin filaments attached to microvilli surface by villin
Actin filaments atttached to basement membrane by spectrin
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Term
| Describe the structure of microvilli |
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Definition
motile hair-like projections that aid particle movement
Composed of axonene or basal body MT arrangement |
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Term
| What is axonene arrangement? |
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Definition
| 2x9 microtubule pairs with a central core of 2 (one pair |
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Term
| What is basal body arrangement? |
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Definition
| 3x9 MT arrangement without a core |
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Term
| What are two types of anchoring junctions? |
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Definition
zonula adherens
macula adherens |
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Term
| What are tight junctions? |
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Definition
| regions of the lateral membrane that prevent cell-cell transport |
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Term
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Definition
| Aggregation of small channels on the lateral surface to encourage cell-cell transport |
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Term
| What are macula adherens? |
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Definition
| Zones of intertwining intermediate filaments shared between neiighboring cells |
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Term
| What are zonula adherens? |
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Definition
| a belth\-like junction that maintains connection with surrounding cells |
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Term
| What is the connective tissue supporting an epithelial gland called? |
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Definition
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Term
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Definition
| the functional unit of an epithelial gland |
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Term
| Whats the difference between exocrine and endocrine glands? |
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Definition
Exocrine glands have a duct connecting gland to surface (lumen)
Endocrine glands have lost connection to surface, secretions released to blood |
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Term
| How can you classify exocrine glands? |
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Definition
MUNDG
- Unicellular vs multicellular
- Nature of secretion
- Mode of secretion
- Duct shape
- Gland shape
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Term
| What are the modes of secretion? Define |
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Definition
Holocrine-secretory cell matures and dies--becomes part of secretion
Merocrine- Secretory vesicle contain secretion fuses with membrane and release product
Apocrine-cell releases cytoplasm-containing secretion
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