Term
| What does a plate count determine? |
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Definition
| The concentration of bacterial cells in a broth culture of bacteria |
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Term
| How can a concentration of bacteria cells in a broth culture be enumerated? |
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Definition
| plating a known volume of cells on agar media |
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Term
1 bacteria cell = ___ bacteria colonies
1 bacteria cell = ___ colony forming unit (CFU) |
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Definition
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Term
| What is the volume being transferred from one tube to another or from a tube to a plate? |
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Definition
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Term
| What is the volume of liquid in broth per dilution blanks prior to making a transfer? |
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Definition
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Term
| What is a series of controlled transfers down a line of dilution blanks? |
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Definition
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Term
| What is the available count range for determining the CFU? |
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Definition
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Term
| What is the equation for determining the CFU/mL? |
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Definition
| (# of bacteria colonies counted)/(vol of cells plated)(FDF of sample tube) |
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Term
| What is the Multiple Tube Fermentation Test also known as? |
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Definition
| Most Probable Number (MPN) |
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Term
| What does the MPN test detect? |
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Definition
| Number of coliforms in a 100mL sample; rough estimate of E.coli |
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Term
| What are the three medias used in the Multiple Tube Fermentation Method? |
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Definition
| Lauryl Tryptose Broth (LTB), Brilliant Green Lactose Bile (BGLB), and EC (E.coli) Broth. |
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Term
| What are the two main ingredients in the LTB? |
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Definition
| Lactose and Lauryl sulfate |
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Term
| What are the ingredients in BGLB? |
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Definition
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Term
| What are the ingredients in E.Coli Broth? |
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Definition
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Term
| What is the temperature of incubation for an E.coli Broth? |
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Definition
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Term
| What type of media is the Endo Agar? |
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Definition
| Selective and Differential |
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Term
| Describe the organism inhibited by Endo Agar. |
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Definition
| Gram positive due to sodium sulfite and basic fuchin dye |
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Term
| What are the results for an Endo Agar that is dark pink or red or has a green metalic sheen? |
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Definition
| Organism ferments lactose (coliforms produced) |
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Term
| What are the results for an Endo Agar that is colorless or light pink? |
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Definition
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Term
| What is the acceptable ratio for a coliform containing substance? |
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Definition
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Term
| What is considered a countable coliform plate? |
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Definition
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Term
| What is the size of the microorganisms trapped by the microcope membrane filter? |
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Definition
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Term
| How many known species of fungi are there? |
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Definition
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Term
| How many of the known species are pathogenic to humans and animals? |
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Definition
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Term
| What are the two categories of fungi that describe their methods of energy harvesting? |
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Definition
| chemoheterotrophs and absorptive heterotrophs |
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Term
| Fungi are _____ (decompose dead organic matter) or _____ on plants and animals |
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Definition
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Term
| What is the study of fungi? |
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Definition
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Term
| What is secreted in order for fungi to degrade organic matter to use the nutrients as an energy and carbon source? |
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Definition
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Term
| What are the two main categories/types of fungi? |
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Definition
| Yeasts (unicellular) and Fungi (Multicellular and Filamentous) |
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Term
| Fungi are nonmotile eukaryotes what are made of what? |
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Definition
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Term
| Fungi decompose dead plant matter by producing ____ and degrading _____ |
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Definition
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Term
| What are fungi that have both yeasts and molds in their lifecycles called? |
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Definition
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Term
| What are filamentous fungi that produce fleshy reproductive structures? Give three examples. |
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Definition
Macrofungi
ex. mushrooms, puffballs, and shelf fungi |
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Term
| Dimorphic Fungi are pathogenic/nonpathogenic |
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Definition
| pathogenic (temperature dependent) |
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Term
| At what temperatures do yeasts and molds of dimorphic fungi grow? |
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Definition
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Term
| What are individual fungal filaments known as? What are they collectively known as? |
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Definition
Hyphae-individual
mycelium-collective |
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Term
| What are the two types of hyphae? |
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Definition
vegetative (obtain nutrients)
reproductive/aerial (bear spores) |
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Term
| Pigmented fungi are called what? |
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Definition
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Term
| What are fungi called that have hyphae consisting of uninucleate cell units with walls that separate adjacent cell? |
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Definition
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Term
| What are fungi called that have hyphae that are continuous cells that contain numerous nuclei? |
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Definition
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Term
| What class of Fungi contains yeasts and molds and has aseptate hyphae? |
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Definition
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Term
| In the class Zygomycota, spores are produced by specialized hyphae called ___ that serve as stalk on which a ___ contains the spores. |
|
Definition
sporangiophores
Sporangium |
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Term
| What do the sexual spores form that are produced when two different mating types produce specialized hyphae that fuse together? |
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Definition
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|
Term
| What is the common name for the Class Zygomycota? |
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Definition
|
|
Term
| What are two examples of the class Zygomycota? |
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Definition
Rhizopus (common black bread mold)
Pilobolus (mold on horse dung) |
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Term
| What class of Fungi contains both molds and yeast and has septate hyphae? |
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Definition
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Term
| What are the specialized hyphae in the Class Ascomycota called? |
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Definition
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Term
| In the class Ascomycota, the zygote undergoes meiosis in the ___ or sac to produce ___ |
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Definition
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Term
| What is an array of asci oriented in elaborate structures called? |
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Definition
|
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Term
| What is the common name for the class Ascomycota? |
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Definition
|
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Term
| What are four examples of the class Ascomycota? |
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Definition
| Truffles, Morel,Peziza repanda, Claviceps purpurea |
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Term
| What is the yeast and class that is responsible for the production of alcohol? |
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Definition
| Saccharomyces cerevisiae; Class Ascomycota |
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Term
| What class of Fungi include mostly molds that have septate hyphae? |
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Definition
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Term
| In the class Basidiomycota, the fusion of two nuclei from different mating pairs occurs within the club-shaped hyphae called ____. |
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Definition
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Term
| In the class Basidiomycota, what two things refer to the fleshy above ground mushroom, puffball, or shelf fungi? |
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Definition
| Fruiting body of the mycelium or basidiocarp |
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Term
| What is the common name for the class Basidiomycota? |
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Definition
|
|
Term
| What are four examples of the class Basidiomycota? |
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Definition
| Stinkhorn, Toadstool, puffballs, shelf fungi |
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Term
| What class of fungi have septate hyphae and reproduce asexually (conidiospores) and resemble ascomycetes? |
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Definition
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Term
| What are two examples of the class Deuteromycota? |
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Definition
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Term
| Unpigmented fungi are called what? |
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Definition
| hyaline/moniliaceous fungi |
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