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| Transfer of dna ino eukaryotic cells is knows as? |
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| which of the following techniques is most common to separate dna by size? |
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| cutting dna with restriction enzymes and analyzing the banding pattern of fragments |
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| The DNA profiling database maintained by the fbi is called? |
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Definition
| CODIS combined dna index system |
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| an enucleated egg cell is? |
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| Which technique for screenng 1000 or more genes? |
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| which of the following genetic disorders is created by translocation between chromosom 9 and chr 22? |
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Definition
| chronic myelogenous leukemia |
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| which of the following would be used to prepare fetal karyotpe to detect trisomy 21? |
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Definition
| chorionic villus sampling |
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Term
| which of the following best describes rnai techniques for gene silencing? |
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Definition
| siRNAs trigger degradation of mRNA to prevent their translation |
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| Gene therapy involves direct injection of DNA into cells within the body |
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Definition
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Term
| small hollow mmbrane vesicles used to deliver theapeutic genes for gene therapy are called? |
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| Dolly sheep was genetically similar to? |
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Definition
| the mother from which nuclear dna was taken |
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| Plasmids are vectors for gene cloning because they? |
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Definition
| self replicate in bacterial cells |
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Term
| why are plants used for drug production? |
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Definition
| inexpensive to grow in large quantities, self-repair more efficiently, methods for gene transfer and expression are well established for this plant because long history of plant breeding |
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Term
| what are the 3 R's in animal research? |
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Definition
Replace: the use of animals with alternative techniques or avoid animals altogether
Reduce: the number of animals to a minimum that are tested.
Refine: the way that experiments are carried out to make sure animals suffer as little as possible. |
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Term
| what are two alternatives to animal models? |
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Definition
1 in vitro cell culture techniques
2 in silico computer simulations |
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| what are shortcoming of alternative animal models? |
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Definition
cultured cells involve animal derived products such as serum
simulations use data from previous animal experiments |
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| why are zebra fish ideal for studies? |
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Definition
| rapid growth visible, eggs accept gene transfer readily, transparent embryos, |
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Term
| Antibody secreting tumors? |
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Definition
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Term
| gene from one organism introduced into another organism to create a trangenic; term usually applies to genes used to create transgenic animals and lpants |
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| an active gene is replaced with DNA that has no functional information |
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| splitting embryos in half at the two cell stage of development to produce two embryos |
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| layer of cells in the blastocyst that develop to form body tissues a source of embryo9neic stem cells |
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| involves a complex cascade of cellular proteins that cause cell death |
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| substances used as indicators of a biologic state objectively measured and evaluated as an indicator of normal processes, patho proc, or pharm responses to therapy intervention |
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Definition
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Term
| a chip consistng of a glass microscop slide containing thousands of pieces of single stranded dna molecules atached to specific spots on the slite; each spot of dna is a unique sequence |
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| hybrid cells used to create monoclonal antibodies; created by fusing B cells with cancerous cells |
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Definition
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Term
| small hollow sphres or particles made of lipids; can be packaged o contain molecules such as DNA and medicines for use in therapeutic procedures ie gene therapy |
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Definition
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Term
| this method introduces the transgene dna at the earliest possible stage of development of the fertilized egg |
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Definition
| pronuclear microinjection |
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Term
| RNA baed mechanisms of gene silencing |
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Definition
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Term
| transplanting a patients own tissues from one region of the body to another;for example coronary artery bypass surgery |
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Definition
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| designing and growing tissues for use in regenerative medicine application |
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| hollow cluter of approximatey 100 cells formed about 1 week after fertilization of an egg |
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| creating an embryo without fertilization by pausing DNA division in an egg and allowing the egg to develop with an increased number of chromosomes; for instance, preventing chromosomes from separating in a human egg creates a diploid cell that may develop to form an embryo. |
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Definition
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Term
| what is fluorescence in situ hybridization? |
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Definition
| cytogenic technique developed by biomedical researchers to use fluorescent probes to bind only to specific sequences on chromosomes for whatever reason |
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Term
| Which does NOT apply to chloroplasts? |
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Definition
found only in green plants? false
synthesize atop from adp? false they make pyruvate |
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Term
| down syndrome is caused by |
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Definition
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| which cell feature processes proteins? |
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Definition
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Term
| colorblindess is sexlink recessive and appears more ofthen in |
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Definition
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Definition
| seperating rna molecules by gel electrophoresis. |
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Definition
| a specific sequence of DNA nucleotides that serves as a unit of inheritance |
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| lab technique that uses single stranded dna or rna probes labeled with fluorescent nuc to identify gene sequences ina chromosome or sel in situ |
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Definition
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| a mutation changing codon for another codon that codes for a dif amino acid |
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Definition
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Definition
| reads same forwards or back |
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Term
| a single base change in dna sequence |
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Definition
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| changing codon into stop codon |
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Term
| process of administering a vaccine? |
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Definition
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Term
| Dna or viruses that can be used to carry and replicate other pieces of dna |
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| a genetically identical copy of a cell or whole organism? |
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| a procedure for binding ssdna probe to dna molecules from bac colonies |
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Definition
| protein degradign enzyme that makes cheese |
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| large circular dsdna vector used for gene cloning in bacteriophages |
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| overhanging ss ends of a dna molecule by re's |
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| endeavors to capitalize on knowledge of natural resource |
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| virus that infects bacteria |
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