Term
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Definition
| copying of genetic material in order to pass it on to a secondary generation |
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Term
| template/parental strands |
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Definition
| original complementary dna strands that are "unzipped" to act as patterns for two new strands of dna |
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Term
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Definition
| two new identical strands of dna generated from parental strands |
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Term
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Definition
| favored theory for method of dna replication; each molecule contains one parental strand and one daughter strand after the first replication |
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Term
| bidirectional replication |
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Definition
| bacterial method for replicating circular chromosomal dna; replication proceeds in both directions from the single origin of replication |
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Term
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Definition
| where DnaA protein binds to in the origin of rep in a bacterial chromosome to initiate replication; causes AT-rich region to separate |
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Term
| oriC (origin of chromosomal replication) |
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Definition
part of E. coli chromosome that contains
1. AT-rich regions
2. DnaA box sequences
3. GATC methylation sites |
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Term
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Definition
| sequences of DNA that are cause to separate when DnaA binds with the DnaA box sequences. because only 2 hydrogen bonds for between A and T, DNA strands are more easily separated in AT-rich regions |
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Term
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Definition
| DnaB proteins; breaks hydrogen bonds between double stranded dna to generate single strands away from the origin of replication in a 5'-3' direction |
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Term
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Definition
| aka topoisomerase II, travels in front of dna helicase loosening supercoils |
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Term
| single-strand binding proteins |
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Definition
| prevents two separated parent strands from readhering and forming a double helix |
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Term
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Definition
| short strands of rna attached to the parental strand by primase to start replication; eventually removed |
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Term
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Definition
| single primer made at the origin of rep, replication begins in 5'-3' direction |
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Term
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Definition
| multiple primers along direction of replication; |
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Term
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Definition
| synthesizes DNA daughter strands, leading and lagging |
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