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| genetic element that can self-replicate and exist indpendently of the bacterial chromosome |
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| genetic factor capable of integrating into the bacterial chromosome |
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| transfer genetic info from one cell to another via a virus |
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| transfer genetic material from one cell to another |
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| random passage of bacterial genes to recipient cells via lytic phage infection |
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| transfer genetic material due to integration of a lysogenic phage into the host genome......eg: lambda bacteriophage |
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| markers close together on a chromosome can be transferred together during lytic infection |
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| dna picked up directly from the medium and recombined in the genome |
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| kills host...and always undergo lytic infection cycle |
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| what are the 2 types of streptococcus pneumoniae? |
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Definition
| 1-Rough (R) and 2-Smooth (S) |
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| nonpathogenic strain.. bacteria unable to synthesize polysaccharide coat. |
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| pathogenic strain.. polysaccharide coat allows cells to escape immune surveillance |
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| biochemically fractionated pneumococcal extracts for R and S found DNA was responsible was the transforming principle. |
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| radioactive label experiment....found 32P was passed to progeny and not 35S. |
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| 3 components of DNA structures? |
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Definition
nitrogenous base
pentose sugar
phosphate group |
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| x-ray diffraction and fourier analysis? |
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| shows images through crystals and printed on film. how they found out about helical structure of DNA |
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| MRC , Cambridge, took picture of helical structure |
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| discovered helical structure of dna |
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| b( human) a(less hydrated) z(left handed helix) |
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| semiconservative (correct model) conservative, and dispersive |
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| labels dna with 3H thymidine, makes picture with x-ray film. and demonstrated that replication happens bidirectionally. |
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| meselson and stahl experiment |
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Definition
| demonstrate that semiconservative replication was correct model. |
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