Term
| List the criteria for selecting appropriate blood for crossmatching |
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Definition
| A negative result indicates compatibility, recipient antibodies have to be given antigen negative donor blood |
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Term
| Select appropriate blood to crossmatch with specimens given |
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Definition
| ABO and Rh compatible with patient’s plasma and antibodies, transfused plasma must be ABO compatible with the recipient red cells. |
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Term
| Correctly perform the crossmatch and interpret the results |
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Definition
| add 2 drops of patients serum to 1 drop of 5% suspension of donor cells, centrifuge for 15 seconds and read for agglutination, add 2 drops of LISS, incubate for 10-30 minutes, centrifuge for 15 seconds and read for agglutination, wash 4 times and add 2 drops of AHG and centrifuge for 15 seconds and read for agglutination, add check cells to negative tubes, centrifuge for 15 seconds, should be positive. |
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Term
| When is an Immediate Spin Crossmatch sufficient? |
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Definition
| An immediate spin crossmatch is sufficient when the patient’s antibody screen is negative AND the patient has no history of clinically significant antibodies. |
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Term
| When is a three phase crossmatch required? |
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Definition
| A three phase crossmatch is required if the patient’s antibody screen is positive OR the patient has a history of a clinically significant antibody |
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Term
| What ABO/Rh blood type should be given in an emergency RBC transfusion when there is no time for a sample to be obtained for a type, screen and crossmatch? |
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Definition
| In an emergency, if there is no time for a sample to be obtained for type, screen and crossmatch the patient should be give type O negative blood. |
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