Term
| Differential Centrifugation |
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Definition
Isolation of particular organelles in bulk amount.
Particles of different sizes travel toward the bottom of the centrifuge tube at different rates. |
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Term
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Definition
| Differential Centrifugation breaks open cells in isotonic buffered solutions by _____? This prevents the rupture of membrane. Helps organelles retain high levels of normal activity. |
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Term
| Protein Methodology: Preperative |
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Definition
| Isolation and purification of particular molecule by a process of removal of other molecules (contaminants) it implies the use of material beyond its isolation. |
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Term
| Protein Methodology: Analytical |
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Definition
| allows an estimate of the molecular diversity present in the preparation. |
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Term
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Definition
Contaminants removed and desired protein retained.
Measured in an increase in specific activity. |
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Term
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Definition
| ration of amount of specific protein/total protein |
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