Term
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Definition
| unexpected ABYs in the serum |
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Term
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Definition
Serum incubated with known AGN RBCs
Allowed to sensitize
Excess serum washing away
AHG added to cause agglutination |
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Term
|
Definition
ABY Screen
Crossmatch
Phenotyping |
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Term
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Definition
| Detects allo or auto ABYs in serum |
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Term
|
Definition
| Mix patient serum with donor RBCs |
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Term
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Definition
| Testing of RBCs with known antisera to determine what ABYs are on the cells |
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Term
| IAT All use ___ to bridge the gap between cells with attached IgG ABYs |
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Definition
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Term
| Most clinically significant ABYs are _____ because they react at 37 degrees celsius and can cross the placenta |
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Definition
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Term
| IgM ABYs typically react only at ____ temperatures |
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Definition
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Term
| 3 times when ABY screen is performed |
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Definition
Prior to transfusion on recipient
Donors
Prenatal |
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Term
True or False:
Don't need poly for prenatal because C' is not usually involved |
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Definition
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Term
| Usually use ___ ___ for ABY screen |
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Definition
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Term
| Usually use polyspecific for ______ |
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Definition
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Term
|
Definition
| Group O cells with known AGN |
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Term
|
Definition
| Reduces incubation time to 5-15 minutes |
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Term
True or False:
Albumin is not used as much since the advent of LISS |
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Definition
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Term
|
Definition
Detect cold IgMs
May see hemolysis from C' |
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Term
|
Definition
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Term
True or False:
If there is a reaction in either screening cells I or II or both then the patient has an ALLOANTIBODY which corresponds to one or more of the AGNs on the screening cells |
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Definition
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|
Term
True or False:
If you run an Auto, which consists of patient's serum and their own cells, and it is positive as well as I and II, the patient has an AUTOANTIBODY |
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Definition
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Term
| 3 False Positive Results in the AHG Technique |
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Definition
Dirty Glassware
Over centrifugation / Over Reading
Cold AutoABY agglutinating cells Before wash |
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Term
| 7 False Negative Results in the AHG Technique |
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Definition
-Inadequate or improper washing of cells
-AHG Reagent non-reactive due to deterioration or neutralization
-AHG reagent not added
-Serum not added in the IAT
-Cell suspension either too weak or too heavy
-Under centrifugation
-Failure to identify weak positive reactions |
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