Term
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Definition
| Mostly made of glycine, proline, 4-hydroxyproline, 5-hydroxylysine. Sequences GLY-PRO-Y and GLY-X-HYP are repeated many times. Contains 3 polypeptide chains. |
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Term
| Collagen is most abundant in the... |
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Definition
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Term
| Three main types of covalent linkages of carbohydrates to proteins |
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Definition
N-glycosyl linkage to asparigine
O-glycosyl linkage to serine and 5-hydroxylysine |
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Term
| Ion Exchange Chromatography |
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Definition
| Separates protein based on charge. |
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Term
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Definition
| separates protein on basis of molecular weight. Large elutes before small. |
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Term
| Ion, Affinity and Gel permeation chromatography all... |
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Definition
| use the same types of beads of gel. |
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Term
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Definition
| separates protein on basis of binding affinity for a specific ligand. Tailored to each protein. A ligand is covalently linked to the column matrix. |
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Term
| Polyacrylamide Gel Electrophoresis |
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Definition
Denaturing- separates on basis of molecular weight
Non-Denaturing- separates proteins on the basis of charge |
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Term
| Capillary Electrophoresis |
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Definition
| Separates proteins on the basis of charge to mass ratio |
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Term
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Definition
| separates proteins on the absis of isoelectric point |
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Term
| 2-dimensional Electrophoresis |
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Definition
| Combines separation of proeins based on molecular weight and isoelectric point. |
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Term
| Sodium Dodecyl Sulfate- Polyacrylamaide Gel Electrophoresis |
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Definition
| Separation based on molecular weight. SDS denatures and coats proteins. SDS is negatively charged thus all proteins migrate based only on mass. |
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Term
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Definition
| Separation based on charge and mass. Proteins can move toward any electrode. |
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Term
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Definition
| Fragment protein-> electrophoresis/chromatography-> Mass Spec. |
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Term
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Definition
| Sequencing from N-terminus |
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Term
| Denaturing agents of Proteins |
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Definition
| Urea, Guanidine Hydrochloride, Sodium dodecyl sulfate |
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Term
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Definition
| Provides a precise three-dimentsional structure of the protein at near atomic resolution. |
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Term
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Definition
| Coil of several fibrils, themselves helical beta sheet of strands perpendicular to a cylindrical axis |
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Term
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Definition
| Protein produced by B cells of the immune system that bind to other proteins. 5 classes. Polypeptide chains are held together by disulfide bonds. |
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Term
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Definition
| Small molecule to which an antibody can bind. |
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Term
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Definition
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Term
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Definition
| Composed of two light chains and two heaby chains. Two antigen binding sites per molecule formed by the variable regions. |
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Term
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Definition
| Allows for crossing the placental membrane |
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Term
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Definition
| highest concentration in plasma. Promotes phagocytosis. lowest mw 150KDalt |
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Term
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Definition
| Mucosal secretion. Gives intial defense aainst virus and bacteria. |
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Term
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Definition
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Term
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Definition
| Plays major role in allergies. mw 190 KDalt. |
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Term
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Definition
| Plasma-first Ab produced in response to foreign antigen. Low levels in mucosa. |
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Term
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Definition
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Term
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Definition
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Term
| Basic Immunoglobin Structure |
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Definition
| 4 constant and 2 variable domains |
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Term
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Definition
| Single Serine in active site. |
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Term
| Example of Serine Proteases |
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Definition
| Blood clotting factor, complement factor, trypsin, chymotrypsin, elastase, plasminogen activators: urokinase and tissue, acrosin, cathepsin |
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Term
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Definition
| Inactive Serine Protease; Proenzyme |
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Term
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Definition
| Clot formation is in balance with clot degredation |
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Term
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Definition
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Term
| Serine Protease Active Site Triad |
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Definition
| Aspartic acid, Histidine, and Serine |
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Term
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Definition
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Term
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Definition
| Globular protein in high concentration in red blood cells. It is composed of alpha and beta polypeptide chains (two subunits of each) |
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Term
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Definition
| Fe2+ forms 6 bonds, 4 with pyrole rings, 1 with a histidine perpendicular to its plane, and 1 with O2. |
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Term
| Hemoglobin Tertiary Structure |
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Definition
| 7 alpha helices in the alpha subunit and 8 Beta helices in the beta subunit |
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Term
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Definition
| O2 carrying protein in the sarcoplasm of skeletal muscle cells similar to Hemoglobin |
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Term
| Eqtn. for binding of O2 to Hemoglobin |
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Definition
Y=pO2/(p50+pO2)
Y=number of binding sites occupied/total number of binding sites |
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Term
| Hemoglobin Binding Saturation Curve |
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Definition
| Sigmoidal; indicates that O2 binding is cooperative |
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Term
| Myoglobin Binding Saturation Curve |
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Definition
| Hyperbolic: indicates simple protein-ligand interaction |
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